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For water and solute transport modelling and quantitative and qualitative water management, a thorough understanding of flow routes is necessary. In this study, we directly measured flow route contributions to surface water discharge and solute loads. The measured flow route contributions were used for calibration and validation of an integrated 3D water and solute transport model. Furthermore, we extrapolated the field scale observations of flow route contributions to the sub-catchment and the catchment scale. Our field scale measurements proved to be essential for understanding dynamics of discharge and water quality at the catchment scale level.