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An integrated geophysical survey was carried out with the aim to investigate hydrodynamics, sedimentology, stratigraphy, pollution, archaeology and navigability of Tiber River (central Italy) lower course. Multibeam, side scan sonar and seismic reflection (Sparker and Pinger sources) data were acquired for a length of 80 km. The accurate location of the survey and the special processing used for the multibeam data gave us a digital elevation model of the river bed with a maximum resolution (in places) of 0.2 m.