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This paper considers effects of paleohydrogeological factors on genesis of oxfordian regional reservoir groundwaters composition. Cycles and stages of hydrogeological history of southern regions of the Ob- Irtysh interfluve of the West-Siberian artesian megabasin were defined. Detailed paleohydrogeological studies of the upper Jurassic sediments were carried out and composition of syngenetic groundwaters was elucidated. Factors affecting variation of groundwaters chemical composition in the course of sedimentations basin evolution were defined.