Reservoir souring is a serious problem that appears normally when seawater is injected into sweet reservoir during secondary recovery programs. The presence of sulfides in produced oil creates various problems for the petroleum industry due to their toxicity.Owing to the fact that the main cause of non-hydrocarbon gas generation is the existence of anaerobic bacteria such as sulfate reducing bacteria (SRB), the main aim in this process has been set to investigating samples pertinent to microbial aggregations of Darquin oil reservoirs for delineating sulfate reduction potential of consumed factors. In addition, temperature effect on microbial process of sulfate reduction and nutrition needed by bacteria is studied in order to be able to anticipate amount of growth of bacteria and non-hydrocarbon gas generation in alternate temperatures. The effect of biocide and base nitrate on anaerobic bacteria growth and different kinds of non-hydrocarbon gas generation has already been discussed. Because of biocide and base nitrate compounds existence in Karoon River, which occasionally results from activities of industrial units, so after mixing with water produced from reservoir, the generation of H2S was anticipated, however the reverse was observed.


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