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The Punta del Este and Pelotas sedimentary basins along the South Atlantic margin of South America have gained significant attention following recent hydrocarbon discoveries in Namibia’s Orange Basin. This study integrates the Punta del Este and Pelotas basins segment through a comprehensive chronostratigraphic analysis using 2D seismic data, well data, and biostratigraphic and isotopic dating. By mapping key seismic horizons, the study enables the identification of main structures and basin correlations. A large set of 2D seismic data was used in conjunction with wells and biostratigraphic data. The seismic-well tie process was carried out, making it possible to position the age markers in the seismic sections. A velocity model was created to migrate the interpreted surfaces from the time domain to depth. From surface mapping, it was possible to identify the main existing structures in the segment, understand the structure that limits the basins and identify the regions with important sedimentation of the Cretaceous interval. In summary, this study highlights the importance of integrated studies in regions such as the Punta del Este Basin - Pelotas Basin, emphasizing the need for multidisciplinary approaches to understand the geological evolution and potentiality of petroleum systems.