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This desk study discusses the potential of utilizing ophiolites in Serbia for energy transition, focusing on natural hydrogen harvesting and CO2 sequestration. It highlights that while investigations have primarily focused on understanding deep oceanic lithosphere and plate tectonics through geochemical and geophysical studies, limited exploration has been conducted on ophiolites as energy source/storage. The Vardar zone in Serbia is identified as a significant area for such studies, with several localities suitable for CO2, and could play a key role in Serbia’s decarbonization, but further feasibility studies are needed to assess the economic viability of using ophiolites for energy purposes. Future research should focus on utilization of geophysical methods, such as reflection seismics that can complement and improve the current understanding of the subsurface structures.