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New single-channel high-resolution seismic reflection and multibeam bathymetry data allow a detailed study of the stratigraphy and tectonics of the underexplored Singapore Strait. The data collected aboard the R/V Galaxea during a dedicated 9-day survey (March 2023) reveal the seabed relief, sub-bottom morphologies, Late Quaternary sediment distribution, and underlying bedrock structure. Data show significant structural and stratigraphic contrasts between the western and eastern regions of the Singapore Strait. The folded and faulted bedrocks commonly observed in the west are associated with the Middle Triassic Jurong and Upper Triassic Sentosa groups. In the eastern region, seismic data reveals a network of buried incised channels that record late Quaternary glacial and interglacial environmental and sea-level changes at the core of the Sunda Shelf. Our results uncover unknown chapters in the evolution of the Singapore Strait and, more specifically, the stratigraphic development of Sundaland.