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In this paper, the application of ground penetrating radar (GPR) for the mapping of near surface tree roots is demonstrated. GPR enables tree roots to be mapped in a non-destructive and cost-effective manner and is therefore a useful prospecting tool in a variety of diverse study fields, including geohydrology, ecology and civil engineering. Case study examples are presented of how GPR can play a role in detecting and quantifying subsurface biomass and also how GPR can be used to discriminate between subsurface roots and man-made utilities.