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Abstract

Summary

In order to improve interpretations of the demosponge biomarker 24-isopropylcholestane (24-ipc), we examine the biosynthesis of side chain alkylated sterols, including 24-isopropenylcholesterol, through functional analysis of sterol methyltransferases (SMTs). We experimentally demonstrate sponge SMTs are capable of multiple rounds of methylation as previously hypothesized. We also identify functional SMTs of probable bacterial origin capable of producing 24-methyl, 24-ethyl, and 24-isopropyl sterols, suggesting bacteria may be an overlooked source of side chain alkylated steranes in the geologic record, including 24-ipc.

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/content/papers/10.3997/2214-4609.202134237
2021-09-12
2024-04-26
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